recombinant murine adiponectin  (GenScript corporation)

Journal: Scientific Reports

Article Title: Obesity reduces the anticancer effect of AdipoRon against orthotopic pancreatic cancer in diet-induced obese mice

doi: 10.1038/s41598-021-82617-2

Figure Lengend Snippet: Effects of AdipoRon and obesity-related factors on the survival of Panc02-Luc-ZsGreen cells. ( A ) qRT-PCR analysis of the expression of AdipoRs and LepRs. ( B ) Effects of AdipoRon on the activation of AMPK, p38 MAPK, ERK1/2 and STAT3. The cells were incubated with 25 μg/ml AdipoRon for the indicated times. The uncropped images are shown in Supplementary Fig. S11-1. ( C ) Cell survival. The cells were incubated with various concentrations of AdipoRon for 2 days. The MTT assay and trypan blue dye exclusion test were used for the measurements of cell survival and viability, respectively. The value for each point represents triplicate measurements. ( D ) Cell morphology. The cells were cultured with 30 μg/ml AdipoRon for 2 days. White arrows indicate the cells showing cytoplasmic swelling with large pieces blebbing from the plasma membrane. ( E ) Effect of z-VAD-fmk on AdipoRon-induced cell growth inhibition. The cells were treated with z-VAD-fmk (50 μM) in the presence or absence of AdipoRon (25 μg/ml) for 2 days. ( F ) Effect of U0126 on AdipoRon-induced cell growth inhibition. The cells were treated with U0126 (10 μM) in the presence or absence of AdipoRon (25 μg/ml) for 2 days. ( G ) Effect of adiponectin on cell survival. The cells were incubated with 10 μg/ml or 20 μg/ml adiponectin (APN) and 25 μg/ml AdipoRon for 2 days. ( H ) Effect of glucose on AdipoRon-induced cell growth inhibition. The cells were cultured in low-glucose (1 mg/ml glucose) or high-glucose (4.5 mg/ml) medium containing 10% FBS in the presence or absence of 25 μg/ml AdipoRon. ( I ) Effect of insulin on AdipoRon-induced cell growth inhibition. The cells were incubated with various concentrations of insulin in high-glucose DMEM containing 10% FBS in the presence or absence of 25 μg/ml AdipoRon. ( J ) Effect of IGF-1 on AdipoRon-induced cell growth inhibition. The cells were incubated with various concentrations of IGF-1 in high-glucose DMEM containing 10% FBS in the presence or absence of 25 μg/ml AdipoRon. Cell survival and cell viability were measured with MTT assay and trypan blue staining, respectively. *P < 0.05, **P < 0.01, ns, not significant.

Article Snippet: Murine recombinant leptin and murine recombinant adiponectin were obtained from PeproTech (Rocky Hill, NJ, USA).

Techniques: Quantitative RT-PCR, Expressing, Activation Assay, Incubation, MTT Assay, Cell Culture, Clinical Proteomics, Membrane, Inhibition, Staining

Journal: International Journal of Molecular Sciences

Article Title: Interaction of Nerve Growth Factor β with Adiponectin and SPARC Oppositely Modulates its Biological Activity

doi: 10.3390/ijms20071541

Figure Lengend Snippet: Summary of analyte binding to adiponectin.

Article Snippet: Recombinant murine full-length adiponectin was purchased from Biovender Laboratory Medicine, Inc. (Bmo, Czech Republic), while recombinant murine globular adiponectin and recombinant human PDGF-BB were purchased from Wako Pure Chemical (Osaka, Japan).

Techniques: Binding Assay

Journal: International Journal of Molecular Sciences

Article Title: Interaction of Nerve Growth Factor β with Adiponectin and SPARC Oppositely Modulates its Biological Activity

doi: 10.3390/ijms20071541

Figure Lengend Snippet: Adiponectin suppressed NGFβ-induced neurite outgrowth and cell swelling. ( A , B ) PC12 cells were treated with increasing concentration of NGFβ either in the presence or absence of full-length adiponectin (fADPN, 1 µg/mL) and globular adiponectin (gADPN, 1 µg/mL). Representative results of the cells (arrowhead: neurite) are shown in A and results (percentage of the cells with axon) from five independent experiments are summarized in B. ( C , D ) PC12 cells were treated with NGFβ (1 ng/mL) either in the presence or absence of full length adiponectin and globular adiponectin (0.1 and 1 g/mL). The ratio of the cell with axon ( C ) and the changes in cell body size ( D ) are determined and summarized from three independent experiments. * indicates the statistically significant difference ( p < 0.05) from NGFβ treatment alone (Cont).

Article Snippet: Recombinant murine full-length adiponectin was purchased from Biovender Laboratory Medicine, Inc. (Bmo, Czech Republic), while recombinant murine globular adiponectin and recombinant human PDGF-BB were purchased from Wako Pure Chemical (Osaka, Japan).

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Interaction of Nerve Growth Factor β with Adiponectin and SPARC Oppositely Modulates its Biological Activity

doi: 10.3390/ijms20071541

Figure Lengend Snippet: Adiponectin suppressed NGFβ-induced neurite outgrowth independently of its receptor activation. ( A ) Expression of AdipoR1 and AdipoR2 mRNA in the rat skeletal muscle (SM), liver and PC12 cells are shown. ( B ) PC12 cells were treated with full length adiponectin or globular adiponectin and the amounts of phosphorylated and total AMPK were determined. Representative results and the ratio of phosphorylated and total AMPK are shown ( n = 5). ( C – E ) PC12 cells were treated with unrelated (un), AdipoR1, AdipoR2 and R1 plus R2 siRNA and ( C ) mRNA expression of AdipoR1 and AdipoR2 are shown. ( D ) The transfected cells were treated with vehicle (cont.), globular adiponectin (1 µg/mL) and full length adiponectin (1 µg/mL) and the state of AMPK activation are shown ( n = 3). ( E ) The transfected cells were treated with vehicle (cont.), NGFβ (1 ng/mL) or NGFβ plus full length adiponectin (1 µg/mL) and the ratios of the cell with axon are shown ( n = 3). The transfected cells treated with vehicle did not induce any neurite (axon) as shown in A and the ratio calculated was 0 as in B. Thus, bar for control value of each siRNA was not seen. * indicates the statistically significant difference ( p < 0.05) from cont. or NGFβ treatment alone.

Article Snippet: Recombinant murine full-length adiponectin was purchased from Biovender Laboratory Medicine, Inc. (Bmo, Czech Republic), while recombinant murine globular adiponectin and recombinant human PDGF-BB were purchased from Wako Pure Chemical (Osaka, Japan).

Techniques: Activation Assay, Expressing, Transfection